Sequence of the lacZ gene of Escherichia coli.
نویسندگان
چکیده
منابع مشابه
Sequence of the ebgA gene of Escherichia coli: comparison with the lacZ gene.
We have sequenced the ebgA (evolved beta-galactosidase) gene of Escherichia coli K12. The sequence shows 50% nucleotide identity with the E. coli lacZ gene, demonstrating that the two genes are related by descent from a common ancestral gene. Comparison of the two sequences suggests that the ebgA gene has recently been under selection. A significant excess of identical, rather than synonymous, ...
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Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
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Four independent nonsense mutations were engineered into the Escherichia coli chromosomal lacZ gene, and reversion rates back to LacZ(+) phenotypes were determined. The mutation potential of bases within putative DNA secondary structures formed during transcription was predicted by a sliding-window analysis that simulates successive folding of the ssDNA creating these structures. The relative b...
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The exact nature of the early steps of the blotin biosynthetlc pathway of E.coli remains a mystery. Both the bloH and bloC gene products have been shown to be Implicated in these early steps but their precise function is unknown (1,2). Here we present the nucleotide and deduced amino acid sequences of the E.coli bioH gene which was cloned from an EcoRI-BamHI total genomic bank, of E^ coli by co...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1983
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1983.tb01468.x